Dog Adenovirus Type 2 Antigen Rapid Test Kit (CAV2) (Latex Immunochromatography)

Product Code:
E-CAV2-01

Packaging Specification:
20 tests/box

Intended Use:
This product is used for the qualitative detection of Canine Adenovirus Type 2 (CAV2) antigen in dog nasal secretion samples, for the initial screening of CAV2 infection.

Background of CAV2 Infection:
Canine Adenovirus Type 2 infection is a highly contagious disease in dogs, causing respiratory, gastrointestinal, and neurological symptoms. The virus is transmitted through direct contact with infected animals or their secretions. Early diagnosis is crucial for timely treatment and to prevent the spread of the virus.

Test Principle:
This reagent adopts the double antibody sandwich method to detect CAV2 antigen. It contains antibodies against CAV2 antigen pre-fixed on the test area (T) of the membrane, latex-labeled antibodies against CAV2 antigen, and secondary antibodies pre-fixed on the control area (C) of the membrane. When an appropriate amount of sample is added to the sample well of the test card, the sample moves along the test card. If the sample contains CAV2 antigen, the latex-labeled antibodies will bind to it, forming an antigen-antibody-latex complex. During the chromatography process, this complex binds to the CAV2 antibodies fixed on the membrane, resulting in a red band appearing in the test area (T). If the sample does not contain CAV2 antigen, no red band will appear in the test area. Regardless of the presence of the virus antigen in the sample, a red band will always appear in the control area (C). If the control line does not appear, the test result is invalid, and the sample should be retested.

Main Components:

• CAV2 Antigen (CAV2 Ag) Test Card
• Sample Diluent
• Disposable Pipette
• Sample Collection Cotton Swab
• User Manual (1 copy)

Storage Conditions and Shelf Life:
The test card should be stored at 4-30°C, in a cool, dark, and dry place. The shelf life is 24 months. The packaging should be opened in an appropriate temperature and humidity environment, and the test card should be used as soon as possible after opening.

Sample Requirements:

• The test uses dog nasal secretion samples, which can be collected using a disposable cotton swab.
• Samples can be stored at 2-8°C for up to 24 hours or at -20°C or lower for longer periods.
• Ensure that the sample temperature is returned to room temperature before use.

Testing Method:

1. Collect nasal secretions with a cotton swab.
2. Place the cotton swab into a sample tube containing 1ml of sample diluent (do not mix sample diluents from different products).
3. Rotate the sample tube containing the cotton swab to mix the sample and diluent thoroughly.
4. Slowly add 80ul (3-4 drops) of the mixed solution to the sample well.
5. During the reaction, a red band will move in the result window of the test strip.
6. Read the result after 15 minutes. Results read after 30 minutes are invalid.
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Interpretation of Test Results:

• Positive: A red band appears in both the test area (T) and the control area (C).
• Negative: Only a red band appears in the control area (C), and no red band appears in the test area (T).
• Invalid: No red band appears in the control area (C), indicating that the test is invalid, and the sample should be retested.
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Precautions:

• Follow the instructions in the manual.
• Do not use tap water, purified water, or distilled water as a negative control.
• The color intensity of the band in the test area (T) may vary. Within the specified observation time, even a very faint band should be considered a positive result.
• Strictly follow relevant biosafety guidelines for sample collection, storage, and testing.
• The test card is recommended to be used immediately after opening and should not be reused.
• The test result is for clinical reference only. Clinical diagnosis should consider symptoms, signs, medical history, and other laboratory test results.
• The desiccant in the product aluminum foil bag is not for consumption.
• Before testing, ensure that the sample and test card have been brought to room temperature. The recommended testing temperature is 18-30°C.