Dog Distemper Virus Antigen Rapid Test Kit (CDV Ag) (Latex Immunochromatography)

Product Code:
E-CDV-01

Packaging Specification:
20 tests per box

Intended Use:
This product is used for qualitative detection of canine distemper virus antigen in canine ocular and conjunctival secretions, nasal discharge, and saliva samples, serving as an initial screening test for canine distemper virus infection.

Canine distemper is a highly contagious disease caused by the canine distemper virus, with a mortality rate of up to 80% or higher. Affected dogs exhibit biphasic fever, rhinitis, severe digestive tract disorders, and respiratory inflammation. A small number of cases may develop encephalitis. The natural hosts of infection are canine and mustelid animals, and the disease is primarily transmitted through air and droplets. The clinical manifestations of the infection are complex and diverse, making clinical diagnosis difficult and prone to confusion with upper respiratory infections, canine infectious hepatitis, and canine coronavirus infection, significantly impacting pet health.

Test Principle:
This reagent employs a double antibody sandwich method to detect canine distemper virus antigen. It contains antibodies against canine distemper virus antigen pre-fixed on the membrane test area (T), latex-labeled antibodies against canine distemper virus antigen, and secondary antibodies pre-fixed on the membrane control area (C). When an appropriate amount of the sample to be tested is added to the sample well of the test card, the sample will move forward along the test card. If the sample contains canine distemper virus antigen, the latex-labeled antibodies will bind to it, forming an antigen-antibody-latex complex. During chromatography, this complex binds to the canine distemper virus antibodies fixed on the membrane, resulting in a red band appearing in the test area (T). If the sample does not contain canine distemper virus antigen, no red band will appear in the test area. Regardless of the presence of viral antigen in the sample, a red band will always appear in the control area (C). If the control line does not appear, it indicates an invalid test result, and the sample should be retested.

Main Components:

• Canine Distemper Virus Antigen (CDV Ag) Test Card
• Sample Diluent
• Disposable Pipette
• Sample Collection Swab
• User Manual (1 copy)

Storage Conditions and Shelf Life:
The test card should be stored at 4-30°C, in a cool, dark, and dry place, with a shelf life of 24 months. The packaging bag should be opened in an appropriate temperature and humidity environment, and the test card should be used as soon as possible after opening.

Sample Requirements:
This test uses nasal discharge, saliva, and ocular conjunctival secretions from dogs, which can be collected using a disposable swab. Samples can be stored at 2-8°C for 24 hours or at -20°C or lower for longer periods. Ensure that the sample temperature is restored to room temperature before use.

Testing Method:

1. Collect ocular conjunctival secretions, nasal discharge, or saliva using a swab.
2. Place the swab into a sample tube containing 1ml of sample diluent (do not mix sample diluents from different products).
3. Rotate the sample tube containing the swab to thoroughly mix the sample and sample diluent.
4. Slowly add 80ul (3-4 drops) of the mixed solution to the sample well.
5. During the reaction, a red band will be observed moving in the result window in the middle of the test strip.
6. Read the result after 15 minutes. Results read after 30 minutes are invalid.
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Interpretation of Test Results:

• Positive: A red band appears in both the test area (T) and the control area (C).
• Negative: Only a red band appears in the control area (C), and no red band appears in the test area (T).
• Invalid: No red band appears in the control area (C), indicating an invalid test result. The sample should be retested.
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Precautions:

• Follow the instructions carefully.
• Do not use tap water, purified water, or distilled water as a negative control.
• The band in the test area (T) may appear with varying degrees of color intensity. Within the specified observation time, regardless of the color intensity, even a very faint band should be interpreted as a positive result.
• Sample collection, storage, and testing should strictly follow relevant biosafety guidelines.
• The test card is recommended for immediate use after opening and should not be reused.
• The test result is for clinical reference only. Clinical diagnosis of the disease should be considered in conjunction with symptoms