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Due to its enhanced sensitivity (1.5x higher), weak signals can be detected with very low noise levels.
An example of the high sensitivity analysis for fluorescein is shown. The F-7100 detected fluorescence in the order of 1x10-13 mol/L (sub-picomol) compared with a blank sample (purified water); a useful calibration was obtained in the ultra-trace range.
With the application of the new Xe lamp and the improved lamp ignition power source, both luminance and the lamp lifetime were increased.
Increased lamp lifetime reduces operating Cost-of-Ownership and instrument service time.
*1Comparison with the service life (recommended replacement interval) of the standard Xe lamp (PN: 650-1500) of F-7000 fluorescence spectrophotometer.
*2Lifetime is usable limit. Guaranty for 500 operating hours or 6 months, whichever shorter.
Troubles due to mishandling are not within guarantee.
The fast-speed scanning enables users to carry out measurements that have been difficult with conventional instruments. In this example, an isomerization process of coumarin in kerosene was traced by spectrum measurement at 2-second intervals and displayed as a 3-D time-scan spectrum. This is a new function in the F-7000.
Previously, a quick reaction which occurs within 1 minute could be measured only by using the fixed wavelength method. The F-7000 is capable of following such a quick reaction because of its fast scanning, measuring the entire wavelength range within 1 second.
The variations in the fluorescence intensity over time and between instruments can be corrected. Fluorescence intensity is affected by changes in lamp brightness, room temperature, optical system, etc. The fluorescence intensity of the standard sample is measured, and the sample fluorescence intensity is converted to the fluorescence intensity relative to the standard sample. This standardization is also used for the intensity comparison between different instruments, including the analysis of humic substances in environmental water (conversion to quinine sulfate), the analysis of chlorophyll in water (conversion to fluorescein), and the specified value for reagent purity (conversion to quinine sulfate).
Item | Description | |
---|---|---|
Sensitivity (Raman light of water) | Noise: Background | S/N 20,000 or above*3 |
Noise: Peak | 1,200 or above*4 | |
Minimum sample volume | 0.6 mL (in use of standard 10 mm rectangular cell) | |
Photometric principle | Monochromatic light monitoring ratio calculation | |
Light source | 150 W xenon lamp, self-deozonating lamp house | |
Monochromator | Stigmatic concave diffraction grating: 900 lines/mm, F2.2 Brazed wavelength: Excitation side 300 nm, emission side 400 nm | |
Measuring wavelength range (on both EX and EM) | 200 to 750 nm, and zero-order light (Expandable up to 900 nm with optional detector) | |
Bandpass | Excitation side: 1, 2.5, 5, 10, 20 nm Emission side: 1, 2.5, 5, 10, 20 nm | |
Resolution | 1.0 nm (at 546.1 nm) | |
Wavelength accuracy | ±1 nm | |
Wavelength scan speed | 30, 60, 240, 1,200, 2,400, 12,000, 30,000, 60,000 nm/min | |
Wavelength drive speed | 60,000 nm/min | |
3D measurement time | 3 min*5 | |
Response | Response from 0 to 98 %: 0.002, 0.004, 0.01, 0.05, 0.1, 0.5, 2, 4 s | |
Photometric value range | -9999 to 9999 | |
Data processing unit | PC : Windows 7 | |
Printer | Printer compatible with Windows 7 | |
Dimensions/weight | Spectrophotometer: 620 W × 520 D × 300 H mm(excluding protrusions), 41 kg | |
Working temperature | 15 to 35 °C, 25 to 80% (condensation not/humidity allowed, 70% or less at 30 °C, or higher) | |
Power consumption | 100, 115, 220, 230, 240 V AC, 50/60 Hz, 380 VA |
*3EX 350nm, Slit 10 nm, Response 4 s
*4EX 350nm, Slit 5 nm, Response 2 s
*5EX 200 to 750 nm, Sampling interval 10 nm, EM 200 to 750 nm, Sampling interval 10 nm