Terminal deoxynucleotidyl transferase, recombinant (rTdT) is a DNA polymerase that catalyzes the transfer of 3fi hydroxyl groups to DNA A reaction in which deoxyribonucleotides are added to the terminal ends. Provide TdT technical bulletins.

Applications: Homopolymer tailing of vectors and inserts for cloning. Oligonucleotides labeled with biotin (1,2), ³²P or ³⁵S (3), or in cells undergoing apoptosis (TUNEL) (4, 5) Medium.

Source: Purified from calf thymus TdT E. coli clone.

Performance and quality testing:Endonuclease, 3´ and 5´ exodeoxyribonuclease and measured incorporation levels.

Unit definition:One unit incorporates 1 nmoL dATP into an acidic precipitable material (within 1 h at 37°C using d(pA)<sub>50 As a primer.

Hazard warning: Toxic; reaction buffer contains potassium cacodylate. Also contains highly toxic chemical: cobalt chloride.
See MSDS. .

Unit reaction conditions:0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6, 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [³</sub> sup>H]dATP and enzyme (0.15 mL, 1 h, at 37°C)