Exosome-Human CD63 Isolation/Detection Reagent purifies CD63+ exosomes from pre-enriched cell culture samples ( Also called extracellular membrane vesicles and multivesicular bodies). These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. Exosomes must be pre-enriched before isolation. This can be achieved by ultracentrifugation or using reagents for rapid and efficient isolation of total exosomes (from cell culture media).

• Obtain high purity CD63+ exosomes
• “See” your sample during processing
• Easily scalable protocols
• Pass in less than 1 hour Detection of Exosomes by Flow Cytometry

Isolating and detecting exosomes is a time-consuming, tedious, non-specific and difficult process. Exosome–Human CD63 Isolation/Detection (from cell culture media) utilizes the well-known Dynabeads™ magnetic separation technology, allowing you to easily purify pre-enriched CD63+ exosomes from cell culture media and then further pass them by flow cytometry , electron microscopy or Western blotting and other techniques to detect purified exosomes.

Detection by Flow Cytometry
One of the main advantages of using magnetic separation technology is that individual free exosomes are too small to be detected by flow cytometry. Purified bead-bound exosomes can be easily visualized by flow cytometry. Monodisperse and relatively large Dynabeads™ (4.5 µm diameter) typically provide clear and unambiguous FFC/SSC in less than 1 hour.

Can "see" your sample
Superparamagnetic Dynabeads™ not only have known sensitivity, reproducibility and stability, but also have a light brown color due to the microbeads , and can “see” your sample during magnetic processing. When the sample tube is placed on the magnetic stand, the exosomes bound to the magnetic beads are pulled to the side of the tube, allowing for easier isolation and purification. Additionally, sample and bead volumes can be easily scaled up or down based on sample volume or downstream application.

Good mixing is critical
To successfully isolate exosomes, be sure to use a mixer with a tilt and rotate mixer to ensure that the beads do not settle in the in the tube. Avoid end rotation for small sample volumes (e.g., 100 µl). See the user manual below for more guidance.