Exosome-human CD81 flux detection reagent (from cell culture) can be used with flow cytometry and/or electron microscopy , detection of CD81-positive extracellular vesicles (ECVs), such as exosomes, in enriched cell culture media. Free exosomes alone are too small to be detected by most flow cytometers. Using Dynabeads™ magnetic separation technology, exosomes can be easily visualized by flow cytometry while bound to the bead surface. 2.7 µm Dynabeads™ magnetic beads provide clear and unambiguous flow analysis.

• Extremely low hands-on time for specific exosome capture and analysis

• Clear and unambiguous flow cytometry analysis in less than 1 hour of hands-on time
< br>• Study the expression of markers for specific exosome subgroups

• Protocol is easily scalable

New research shows that the exosome/ECV population consists of several subpopulations that may have different function. Using immunoaffinity-based Dynabeads™ magnetic separation technology, these subgroups can be selectively captured and studied using flow cytometry and/or electron microscopy. Dynabeads™ technology avoids material loss due to handling and centrifugation, a problem commonly encountered with other centrifugation and analytical methods.

Exosomes in the cell culture medium must first be enriched. This can be done quickly and efficiently using Total exosome isolation reagent (from cell culture medium) or traditional ultracentrifugation completed.

Note: Be sure to mix thoroughly to ensure the magnetic beads do not settle in the sample tube. We recommend a mixer that offers tilt and rotation for optimal mixing.