Dynabeads™ CD4 are superparamagnetic microbeads covalently coupled to anti-human CD4 antibodies that can be obtained directly from whole blood, Easily isolate or remove human CD4⁺ T cells from any sample including bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads™ CD4:

• Rapid and efficient isolation of CD4⁺ T cells from any sample—no columns required
• Positive isolation of CD4⁺ T cells have high purity, yield and survival rate
• CD4⁺ T cells can be quickly and efficiently removed from any sample

Information about Dynabeads™ CD4
Dynabeads™ CD4 are uniformly sized superparamagnetic microbeads (4.5 µm in diameter) coated with primary monoclonal antibodies against CD4, which is mainly expressed on human helper T cells Membrane antigens are specific. Due to the size of the beads, Dynabeads™ CD4 can easily and efficiently separate or remove cells from viscous samples such as whole blood and bone marrow in approximately 30 minutes. Dynabeads™ CD4 also enables the efficient recovery of highly pure and viable cells for downstream molecular studies; for example, cells are lysed but remain attached to the beads and further purified nucleic acids or proteins. Please note that intact cells will not be released from these beads, so if you want to isolate CD4⁺ cells for cell-based applications, or you need to examine your sample with flow cytometric analysis, Please view our company's Human CD4 product supply.

Magnetic bead-based separation technology for easy operation
Add Dynabeads™ CD4 to the sample under continuous mixing to optimize the binding of Dynabeads™ to target cells . Place the sample on the magnetic stand and separate the bead-bound target molecules from the rest of the sample in just 1–2 minutes. To remove, transfer the supernatant to a new tube for further study and discard the bead-bound cells. When performing positive isolations for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to obtain ideal purity. Cells can be lysed but still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples include whole blood, buffy coat, bone marrow, PBMC, or tissue digest products.

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