Dynabeads™ CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibodies, which can be directly collected from whole blood, erythrocyte sedimentation rate Buffy coat or MNC suspension separates or removes CD45⁺ leukocytes. Dynabeads™ CD45 can also be used to enrich epithelial tumor cells. CD45 antigen is weakly expressed on myeloid cells. For efficient removal or isolation of all leukocytes, including myeloid cells, use Dynabeads™ CD15 (Cat. No. 11137D) in combination with Dynabeads™ CD45. Advantages of Dynabeads™ CD45:

•Efficient depletion of human leukocytes
•Isolation of leukocytes directly from whole blood for molecular applications
•Enrichment of circulating epithelial tumor cells

Information about Dynabeads™ CD45
Dynabeads™ CD45 are uniformly sized superparamagnetic microbeads (4.5 µm diameter) coated with primary monoclonal mouse IgG_2a Antibody specific for a common CD45 membrane antigen for all known CD45 isoforms. CD45 is expressed on all human leukocytes. Due to the size of the beads, Dynabeads™ CD45 can easily and efficiently separate or remove cells from viscous samples such as whole blood and bone marrow in approximately 30 minutes. Dynabeads™ CD45 is commonly used for enrichment of circulating non-hematopoietic tumor cells by removing all CD45⁺ leukocytes from MNC samples. Positively isolated cells can be used for downstream molecular studies; for example, cells are lysed but remain attached to beads and further purified nucleic acids or proteins. Please note that intact cells are not released from these beads, so we do not recommend using flow cytometry to analyze bead-bound cells.

Magnetic bead-based separation technology for easy operation
Add Dynabeads™ CD45 to the sample under continuous mixing to optimize the binding of Dynabeads™ to target cells . Placing the sample on the magnetic stand takes only 1–2 minutes to separate the bead-bound target molecules from the rest of the sample. To remove, transfer the supernatant to a new tube for further study and discard the bead-bound cells. When performing positive isolations for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to obtain ideal purity. Cells can be lysed but still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples include whole blood, buffy coat, bone marrow, PBMC, or tissue digest products.

Learn more about Dynabeads™ products
• Get Dynabeads™ products for a variety of applications.
•Get Magnetic Stands for Dynabeads™ Separation< /a>.