Use this kit to isolate pure and viable unexposed B cells from PBMC by negative isolation. This kit removes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and red blood cells. Negatively isolated human B cells remain in the sample and are not in contact with Dynabeads. The microbeads and antibody-free B cells are truly contact-free and appear to be in perfect shape for any functional assay and application.

• High purity, high recovery and viability of human unexposed B cells
• Easy to use and amplify
• No columns required

Convert anti-non-B cell antibodies The mixture is added to the sample and allowed to bind to the cells. Dynabeads™ are added and bind to antibody-labeled cells during a short incubation period. Bead-bound cells on the magnetic rack are rapidly detached and discarded. The remaining negatively separated and unexposed human B cells can be analyzed directly in a flow cytometer and used in any application.

Starting sample
: PBMC