Minimum Essential Medium (MEM) is a commonly used cell culture medium. MEM can be used to culture a variety of suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes wait. Gibco™ MEM Modified Medium is available in a variety of components for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
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MEM was developed by Harry Eagle based on his earlier formulation of Basic Eagle Medium (BME). Many other modifications of MEM have since been made, including Glasgow's MEM, MEM alpha, DMEM, and Temin's modified medium. This MEM formulation is calcium-free and can be used in suspension cultures. This MEM formulation contains Earle's salt for use in CO₂ incubators. This product is made from Earle's Balanced Salt.

Intended Use of Product
For use in in vitro diagnostics. Note: Not for therapeutic use in humans or animals. Use other than as indicated on the product may violate local regulations.

Dual-site cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Gibco™ MEM product (11380-052) manufactured in our Scottish factory. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

MEM does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.