Dynabeads™ Untouched™ Mouse CD4 Cells contain a cocktail of magnetic beads and antibodies to remove all White blood cells, retaining only target mouse CD4⁺ cells. This negative isolation of unexposed mouse CD4⁺ cells is highly pure and active and can be used in any downstream application.
•Non-exposed mouse CD4⁺ cells can be quickly isolated – no column steps required
• High-purity, high-viability mouse CD4⁺ T cells can Ready for any downstream application

Recovered cells with excellent yield, purity and viability
Mouse Depletion Dynabeads™ suspensions contain uniform superparamagnetic microbeads ( 4.5 µm diameter) to easily separate cells from any single cell suspension. Therefore, this reagent is particularly suitable for enriching tissue digests, such as mouse spleen and lymph node cells. Mouse Depletion Dynabeads™ are coated with secondary polyclonal antibodies conjugated to rat IgG. The antibody cocktail contains a mixture of rat IgGs that bind mouse CD8⁺ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, red blood cells, and granulocytes. First add the antibody mixture to the sample tube to bind to unwanted non-CD4⁺ cells. Cells are then washed to remove filtered antibodies and mouse Depletion Dynabeads™ are added. After a short incubation, use Magnet Stand at 1 – Separates bead-bound cells from unbound cells within 2 minutes. Purified target mouse CD4⁺ cells in the supernatant can be transferred to new tubes for any downstream application. This fast and gentle separation method does not require the use of columns and helps ensure high purity, recovery, and viability of unexposed CD4⁺ cells. This protocol can be easily scaled up based on your sample size.

For scientific research use only. Not intended for animal or human diagnostic or therapeutic use.