Glasgow's MEM (GMEM), originally developed by Ian McPherson and Michael Stoker, is a modified form of Eagle's Minimum Essential Medium. It is used to study genetic factors that influence cell capabilities. Glasgow MEM was developed for use with renal cell lines such as BHK-21.
The Glasgow's MEM has been improved as follows:Includes | Does not include |
•L-Glutamine | •HEPES: |
Provide complete
Formulation.
Use Glasgow's MEM. Glasgow's MEM differs from other media in that it contains twice the concentration of amino acids and vitamins than the original basal Eagle's medium and is used without serum. Glasgow MEM is initially prepared in 10% trypsin-phosphate broth. Glasgow's MEM contains no proteins, lipids or growth factors. Therefore, Glasgow MEM requires supplementation with 10% trypsin and phosphate broth. Glasgow MEM uses a sodium bicarbonate buffer system (2.75 g/L) and therefore requires a 5–10% CO
2 environment to maintain physiological pH.
Dual cGMP manufacturingGlasgow MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Glasgow MEM product (21710-025) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.