Ham's F-12 Nutrient Blend (F-12) Blood-free list of cells designed for use with Chinese Hamster Ovary (CHO) cells decking. Since then, F-12 has been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. A variety of F-12 modified media are available for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
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Uses of F-12
F-12 contains a wider range of ingredients than other basal media, including zinc, putrescine, hypoxanthine, and thymidine . F-12 contains no protein or growth factors. Therefore, F-12 requires additives, usually 10% -Culture/fbs.html">Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP Production and Quality Systems
To ensure supply chain continuity, we produce the F-12 at two separate facilities; one in Grand Island, New York; Located in Scotland, UK. Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.