DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that can be used to support many types of mammalian cells grow. Cells that have been successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a wide range of DMEM modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
Complete Formulation.

Using DMEM
DMEM differs from other media in that it contains 4 times the concentration of amino acids and vitamins equivalent to the original Eagle Minimum Essential Medium. DMEM is initially formulated with low concentrations of glucose (1 g/L) and sodium pyruvate, but is often used in high glucose environments with/without sodium pyruvate. DMEM contains no proteins, lipids or growth factors. Therefore, DMEM requires supplements, usually 10% Fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires an environment of 5–10% CO₂ to maintain physiological pH.

cGMP Manufacturing and Quality Systems
DMEM is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, Life Technologies also offers the same DMEM product (41965-039) produced in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.