This DMEM/F-12 modification is as follows:
Complete Recipe.

Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Gibco™ cell culture media in powder form requires supplementation with sodium bicarbonate, pH adjustment, and filtration (for details, see Protocol).

cGMP Manufacturing and Quality Systems
DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer an equivalent DMEM/F-12 product (42400-010) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.

Contains	Does not contain
•L-glutamine	•Sodium bicarbonate
•HEPES:
• Phenol Red