Thermo Fisher 12400024 10 x 1 L DMEM/F-12,powder,HEPES
$224.82 / Parcel$234.26 Min.order：1
12400024 10 x 1 LDMEM/F-12, powder, HEPES
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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium , can be used to support the growth of many different types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
This DMEM/F-12 modification is as follows:
Complete Recipe.
Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Gibco™ cell culture media in powder form requires supplementation with sodium bicarbonate, pH adjustment, and filtration (for details, see Protocol).
cGMP Manufacturing and Quality Systems
DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer an equivalent DMEM/F-12 product (42400-010) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.
This DMEM/F-12 modification is as follows:
Complete Recipe.
Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Gibco™ cell culture media in powder form requires supplementation with sodium bicarbonate, pH adjustment, and filtration (for details, see Protocol).
cGMP Manufacturing and Quality Systems
DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer an equivalent DMEM/F-12 product (42400-010) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.
| Contains | Does not contain |
| •L-glutamine | •Sodium bicarbonate |
| •HEPES: | |
| • Phenol Red |