DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium , can be used to support the growth of many different types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use
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This DMEM/F-12 modification is as follows:Complete
Recipe.
Use DMEM/F-12DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10%
Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO
2 environment to maintain physiological pH. Gibco™ cell culture media in powder form requires supplementation with sodium bicarbonate, pH adjustment, and filtration (for details, see
Protocol).
cGMP Manufacturing and Quality SystemsDMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer an equivalent DMEM/F-12 product (42400-010) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.
Contains | Does not contain |
•L-glutamine | •Sodium bicarbonate |
•HEPES: | |