Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) Yes A widely used basal medium used to support the growth of many types of mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. For a wide range of cell culture applications, we offer a variety of Gibco™ DMEM/F-12 modified media. Use Medium Selection Tool Find the right recipe.
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Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formula combines the high concentration of glucose, amino acids and vitamins in DMEM with various components of F-12.

Use DMEM/F-12
DMEM/F-12 does not contain proteins, lipids or growth factors. Therefore, DMEM/F-12 requires supplementation, usually 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Gibco™ cell culture media in powder form requires supplementing sodium bicarbonate, adjusting pH, and filtering (for details, see protocol).

cGMP Manufacturing and Quality Systems
Gibco™ DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain stability, we also offer an equivalent Gibco™ DMEM/F-12 product (32500-035) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.