pcDNA™ vectors enable high-level constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector has the following key features:

•Promoterless version of the pcDNA™6.2⁄V5-DEST vector (Cat. No. 12489027)
•attR site for Gateway™ cloning
•Compatible with MultiSite Gateway™ Pro kit (e.g., Cat. No. 12537100)
•C-terminal V5 tag for rapid detection
•For blasticidin resistance genes For efficient and stable screening
•Ampicillin resistance gene and pUC replication region sequences for E. coli selection and maintenance

Gateway™ Cloning
To meet all your expression needs, Invitrogen offers advanced Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells and for the generation of native proteins or N- or C-terminal fusion proteins. Whether entry or Ultimate™ ORF clones, all Gateway™ destination vectors have anattR site for recombination using anyattL-side fragment. The table below lists a range of available destination vectors.

Additional materials required, available for purchase separately:Gateway™ Starter Clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.