Invitrogen Platinum II Hot Start PCR Green Master Mix (2X) contains Platinum II Taq hot start DNA polymerase, Platinum II PCR Ready-to-use master mix of buffers and dNTPs for quick and easy preparation of PCR reaction systems. In addition, this master mix also contains 2 tracking dyes for direct gel loading of PCR products. Platinum II Taq Hot Start DNA Polymerase uses a universal annealing temperature, a unique combination of innovative buffers, engineered high-performance Taq DNA polymerase, and outstanding hot start technology to achieve excellent PCR quickly and easily result. Features of Platinum II Taq Hot Start DNA Polymerase include:
• Innovative Buffer—Contains stable primer-template complex components, allowing a universal primer annealing temperature of 60°C to be used in different PCR reactions< br> • Genetically engineered Taq DNA polymerase—achieves rapid amplification of 15 s/kb while tolerating common inhibitors
• Platinum hot-start technology—enablessures superior specificity, sensitivity, and yields; allows for room temperature reaction setup
• Platinum hot start technology—has ultra-high specificity, sensitivity, and yields; allows for room temperature reaction setup Platinum II Taq Hot Start DNA Polymerase is an enzyme engineered to better tolerate PCR reaction inhibitors in sample materials or DNA purification steps. This enzyme has a faster rate of DNA synthesis, often achieving PCR results more than twice as fast as using other Taq DNA polymerases. The patented Platinum Taq antibody hot-start technology inhibits the enzyme activity at room temperature. The enzyme activity is activated only when the antibody modification is detached in the initial denaturation step of 94°C. This automatic "hot start" technology improves the sensitivity, specificity and yield of the reaction, and provides convenience for preparing the PCR reaction system at room temperature. Due to the unique formula of Platinum II PCR buffer, primer pairs designed according to conventional primer design rules can anneal at the universal annealing temperature of 60°C, greatly reducing tedious optimization steps. Co-stabilizing components in the PCR buffer can enhance the stability of the primer-template complex during the annealing process and achieve higher specificity without the need to optimize the annealing temperature for each primer pair. Using Platinum II Taq hot-start DNA polymerase, different PCR reactions can be amplified simultaneously using the same protocol, using a universal annealing temperature and extension time calculated based on the longest fragment, to amplify multiple target fragments simultaneously. Platinum II Taq Hot Start DNA Polymerase provides an optimized Platinum GC Enhancer for amplification of samples with high GC content, improving amplification specificity and yield. Platinum II Taq Hot Start DNA Polymerase can be used to amplify DNA from complex genomic, viral and plasmid templates, as well as for RT-PCR applications such as genotyping, high-throughput PCR or samples of poor purity. In addition, we also provide dye-free master mixPlatinum II Hot-Start PCR Master Mix (2X)< /a> form. Learn more www.thermofisher.cn/platinumiitaq›