Thermo Fisher 16161 1000 reactions Pierce GaussiaLuciferase Glow Assay Kit
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16161 1000 reactionsPierce Gaussia Luciferase Glow Assay Kit
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Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit is especially useful in Gaussia< /i>-Dura luciferase reporter gene provides an extremely bright and stable bioluminescent signal (half-life greater than 1 hour).
Gaussia Features of Luciferase Glow Assay Kit:
•Sensitive—for Gaussia Highly sensitive detection of luciferase activity
• Stable—higher signal stability
• Simple—no need to use a photometer equipped with a sample injector Design
• Compatible—Assay reagents compatible with otherGaussia luciferase
• Suitable for automation—Suitable for use with Qualcomm Quantity Assay
• Convenience—Contains a universal cell lysis buffer and optimized glow assay reagents
• Safety—Allows the user to perform non-radioactive Assay
The Pierce Luciferase Glow Assay Kit contains reagents for the determination of Gaussia luciferase activity in mammalian cell culture media and lysates. When used with the Thermo Scientific Gaussia-Dura Luc vector, this kit provides a very sensitive bioluminescent reporter assay system for secretory or intracellular detection of luciferase activity. Provides higher signal stability. The light output produced by the luciferase reaction can be related to the amount of luciferase protein produced, which in turn is directly proportional to the activity of the promoter driving luciferase expression. This kit can be used to detect the activity of anyGaussialuciferase or derivative using coelenterazine as a substrate.
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Gaussialuciferase and a luminometer or other instrument capable of monitoring luminescence (such as Thermo Scientific Luminoskan Ascent and Varioskan flash microplate readers).
Applications:
• Promoter studies for analysis of cis regulatory elements and trans acting factors< br>• Drug screening
• siRNA and miRNA screening
• Multi-pathway assays to study off-target effects
• Secretory pathway/protein localization reporter gene assay
• Signal transduction pathway analysis
• RNA Splicing Studies
Gaussia Luciferase is a 20kDa protein from the marine copepod Gaussia princeps. Bioluminescent enzymes are highly secreted in cell culture media and enable live cell monitoring of reporter gene activity. The light output produced by the luciferase reaction can be correlated with the amount ofGaussialuciferase protein produced and used to measure the activity of the promoter driving Gaussia expression. The signal generated by Gaussia luciferase exhibits strong flash kinetics and is higher than that of firefly or Renilla luciferase measured under similar conditions Much more.
The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc vector is derived from the wild-type Gaussia gene and is available Same bright but more stable light output. The Gaussia Glow Detection Kit reagents produce glow-type kinetic Gaussia luciferase reactions and are recommended for use in photometers without injectors or where batching is required Applications for processing samples.
Gaussia Features of Luciferase Glow Assay Kit:
•Sensitive—for Gaussia Highly sensitive detection of luciferase activity
• Stable—higher signal stability
• Simple—no need to use a photometer equipped with a sample injector Design
• Compatible—Assay reagents compatible with otherGaussia luciferase
• Suitable for automation—Suitable for use with Qualcomm Quantity Assay
• Convenience—Contains a universal cell lysis buffer and optimized glow assay reagents
• Safety—Allows the user to perform non-radioactive Assay
The Pierce Luciferase Glow Assay Kit contains reagents for the determination of Gaussia luciferase activity in mammalian cell culture media and lysates. When used with the Thermo Scientific Gaussia-Dura Luc vector, this kit provides a very sensitive bioluminescent reporter assay system for secretory or intracellular detection of luciferase activity. Provides higher signal stability. The light output produced by the luciferase reaction can be related to the amount of luciferase protein produced, which in turn is directly proportional to the activity of the promoter driving luciferase expression. This kit can be used to detect the activity of anyGaussialuciferase or derivative using coelenterazine as a substrate.
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Gaussialuciferase and a luminometer or other instrument capable of monitoring luminescence (such as Thermo Scientific Luminoskan Ascent and Varioskan flash microplate readers).
Applications:
• Promoter studies for analysis of cis regulatory elements and trans acting factors< br>• Drug screening
• siRNA and miRNA screening
• Multi-pathway assays to study off-target effects
• Secretory pathway/protein localization reporter gene assay
• Signal transduction pathway analysis
• RNA Splicing Studies
Gaussia Luciferase is a 20kDa protein from the marine copepod Gaussia princeps. Bioluminescent enzymes are highly secreted in cell culture media and enable live cell monitoring of reporter gene activity. The light output produced by the luciferase reaction can be correlated with the amount ofGaussialuciferase protein produced and used to measure the activity of the promoter driving Gaussia expression. The signal generated by Gaussia luciferase exhibits strong flash kinetics and is higher than that of firefly or Renilla luciferase measured under similar conditions Much more.
The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc vector is derived from the wild-type Gaussia gene and is available Same bright but more stable light output. The Gaussia Glow Detection Kit reagents produce glow-type kinetic Gaussia luciferase reactions and are recommended for use in photometers without injectors or where batching is required Applications for processing samples.
For Research Use Only. Not for use in diagnostic procedures.
