• Provides high resolution for protein identification, biomarker discovery and systems biology
• Unique loading capabilities enable extremely high sensitivity in LC/MS
• Designed for TFA-free LC-MS to minimize ion suppression
• Optimized for ESI/MS and MALDI-MS coupling
• Highest column-to-column reproducibility
• Easy to use, cutting-edge miniaturized HPLC
• Pre-installed nanoViper column inlet connector for easy column installation

Main advantages of nanoViper connectors

• Easy to use, small capillary tube without sleeve
• No tools required for assembly
• No damage caused by over-tightening Column: Can withstand pressures up to 1000 bar, compatible with third-party valves and unions
• No test failure due to improper connections

Ideal for trypsin High-resolution peptide mapping of peptides, natural and synthetic peptides, and LC-MS/MS peptide mapping for protein identification, biomarker discovery and systems biology.

• High capacity
• Used to analyze low-abundance peptides often present in complex proteomic samples
• Based on throughput, sensitivity and Amount of sample available, select column length and ID
• Available in 50, 150, 250, 500 mm lengths and 50, 75, 300 and 1000µm ID
• Excellent proteins and peptides separation.
• Trifluoroacetic acid (TFA)-free LC/MS to increase MS sensitivity and minimize suppression effects
• Excellent separation efficiency
• Excellent separation and recovery
• Easy installation with nanoViper™ finger-tight fittings

PepMap nanoscale columns are also available pre-installed with Thermo Scientific™ Dionex™ nanoViper™ finger-tight fittings. Connect to 1000 bar without dead volume.

• No tools required
• Almost zero dead volume connection
• Withstands pressures up to 1000 bar

About Nano, Capillary and Micro Acclaim PepMap Columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analysis . Often, there are limitations on sample volume, and smaller inner diameter columns can improve sensitivity when sample is limited. While nanoLC columns are commonly used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or advancing LC-MS ligation. Thermo Scientific Acclaim PepMap columns offer nano, capillary and micro columns in a variety of chemistries for peptide and protein analysis.

PepMap Trap columns can be used to desalt and preconcentrate peptides prior to liquid chromatography separation with mass spectrometry detection. Capture columns provide maximum efficiency for 1D peptide mapping experiments and 2D-LC analysis.