DNase I (amplification grade) enzymatically cleaves single- and double-stranded DNA into oligodeoxyribose nuclei containing 5' phosphates glycosides. DNase I (amplification grade) is suitable for the elimination of DNA during critical RNA cleanup procedures such as those prior to RNA-PCR amplification. After purification and testing, the level of RNase contamination was undetectable. RNase freedom was determined by ribonuclease assay using RNA molecular weight standards.

Applications
Removal of DNA from RNA and protein preparations.

Specific activity
Specific activity> 10,000 units/mg.

Source
Purified from bovine pancreas

Performance and quality testing
Determine the use of RNA molecular weight standards Ability to perform RNase detection and enzymatic cleavage of single- and double-stranded DNA into oligonucleotides.

Unit Definition
One unit of enzyme can be increased at a rate of 0.001 A₂₆₀ units/min./mL reaction mixture at 25°C Absorbance of high molecular weight DNA solutions.

Unit reaction conditions
0.1 M sodium acetate (pH 5.0), 5 mM MgCl₂, 50 µg/mL calf thymus DNA and 1 mL enzyme, 10 min at 25°C.