Thermo Scientific Pierce Streptavidin Poly-HRP Conjugate is a polymer with horseradish peroxidase Conjugated biotin-binding proteins enable signal amplification and detection of biotinylated antibodies for IHC and other methods.

Strepavidin Poly-HRP Features:

•High Sensitivity—Detects low levels of target (low picogram-femtogram range ) and high signal-to-noise ratio
•Robust—Consistent production and purification that eliminates low or uncoupled molecules for minimal background and high sensitivity
• Flexible—Compatible with chromogenic, fluorescent, and chemiluminescent substrates
•Universal—Compatible with ELISA, Western blotting, and immunohistochemistry (IHC) techniques
•< b>Easy to use—Direct replacement for immunoassays and other assays
•Convenience—Ready-to-use stable liquid format stored at 4°C
• < b>Cost-Effective—less conjugate/detection and standard HRP conjugate required

Strepavidin Poly-HRP Conjugate is designed for use in sample volumes Limited or low levels of target molecules provide higher sensitivity and low background. Purification of streptavidin poly-HRP to remove unbound streptavidin molecules that can reduce signal intensity by competing with horseradish peroxidase (HRP)-conjugate molecules for binding sites . Additionally, the conjugate does not contain HRP monomers that can cause background signal. Pierce Streptavidin Poly-HRP is compatible with chromogenic, fluorescent, and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC), and nucleic acid hybridization analyses.

There are several forms of streptavidin-HRP conjugates that do not contain polymeric HRP (poly-HRP) and are suitable for most biotin detection assays. These and other commonly used HRP-conjugated antibodies and biotin-binding proteins are limited to 1 to 3 HRP molecules per protein to maintain functionality of the conjugate. The molar ratios of this conjugate are useful for routine experiments, but may lack the sensitivity to detect targets at low picogram to femtogram levels without using additional steps to amplify the signal.

When a higher degree of signal amplification is required with standard HRP conjugates, peroxidase antiperoxidase (PAP) systems or avidin-biotin complex (ABC), etc. can be used method. However, these methods increase the cost of the assay and the number of steps that must be optimized. Pierce Strepavidin Poly-HRP circumvents these issues because it uses a polymer of HRP molecules conjugated to strepavidin in a proprietary manufacturing process that maintains functionality while maintaining The molar ratio of HRP in the conjugate was increased. Increasing the molar ratio of HRP molecules to streptavidin allows more HRP molecules to react with the substrate in each binding event. Therefore, Pierce Streptavidin Poly-HRP provides substantial signal amplification without additional steps and saves time and expense compared to other HRP conjugates.

As with all immunoassays, optimization is required to achieve the best signal-to-noise ratio. Switching to higher sensitivity substrates and conjugates can reveal more of the desired signal and background signal. If this occurs, follow standard primary and secondary antibody dilution techniques or perform blocking buffer optimization.