Thermo Scientific Clean-Blot IP Assay Reagent is a horseradish peroxidase conjugate optimized for use in a Anti-immunoprecipitation post-Western blot detection without interference from denatured IP antibody fragments.

Clean-Blot IP reagents allow reliable Western blot detection of target proteins after IP assays (immunoprecipitation). They work by specifically binding functional primary antibodies (whole IgG) without binding to IP antibody fragments, often accompanying immunoprecipitation of the protein by electrophoresis and membrane transfer. Clean-Blot IP reagents and kits eliminate detection interference in antibody heavy chain (approximately 50 kDa) and light chain (25 kDa) IgG fragments for use in initial immunoprecipitation assays.

Clean-Blot IP Detection Reagent Features:

•Universal—binds and detects most IgG subclasses and isotypes of primary antibodies commonly used in Western blotting type substance
• Compatible—valid for use with IP assays using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
• Extremely Cost-Effective—Eliminates the cost and extra work associated with covalently immobilized IP antibodies as a means of overcoming Western blot interference
• Flexible—for chemiluminescence, fluorescence, or colorimetry Horseradish Peroxidase (HRP) Reagents for Substrate Detection
• Easy to Use—No changes to the Western blot protocol required; simply use Clean-Blot IP Detection Reagents instead of traditional secondary HRP coupling Materials
• Hub-free Assay—Clear Western blot results for immunoprecipitation assays without significant interference from denatured IgG bands

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Clean-Blot™ IP Detection Kit (HRP)