Thermo Scientific Pierce Agarose ChIP Kits provide a comprehensive set of reagents and a simple, fast, and reproducible protocol to perform Chromatin immunoprecipitation (ChIP) assay.

Agarose ChIP Kit Features:

•Simple and fast ChIP assay protocol (<8 hours)
•Efficient nuclear isolation and lysis
•Simple and reliable Reproducible enzyme digestion
•Low background and high binding capacity Protein A/G agarose resin
•Highly specific positive control reagents include: RNA polymerase II antibody and GAPDH PCR primer
•Quick and reproducible spin column specifications
• High recovery DNA purification
• Antibodies validated for ChIP available

Pierce Sepharose ChIP Kits contain sufficient reagents to use optimized Protocol and convenient spin column format for performing 30 ChIP assays using appropriate controls. We also offer quality-assured antibodies validated for ChIP for use with Pierce Agarose ChIP Kits. Because antibodies suitable for Western blotting may not work in ChIP assays, we have assembled the most widely available monoclonal and polyclonal antibodies validated for use in ChIP to facilitate the progression from experimental setup to results. Faster.

The advantage of the ChIP assay is its ability to capture snapshots of specific protein-DNA interactions in living cells and then quantify the interactions using standard or quantitative PCR. Successful ChIP assays require several critical steps (cross-linking, chromatin preparation, immunoprecipitation) before detecting the target genomic DNA sequence. Optimizing each step in a ChIP assay protocol individually can be time-consuming. Pierce Agarose ChIP Assay Kits simplify the process, making it easier to obtain accurate and reproducible results.

Pierce Agarose ChIP Kit is used for ChIP detection to stabilize the protein-DNA complex before extraction. Formaldehyde is often used to achieve in vivo cross-linking. Cross-linking directly in cells locks protein-DNA complexes, trapping these unstable and sometimes transient interactions. To cleave, extract, and solubilize cross-linked complexes, the ChIP Assay Kit includes the Thermo Scientific Chromatin Preparation Module (also sold separately, Product No. 26158). In summary, this complete ChIP assay kit provides a simple, reliable, and convenient method for isolating chromatin-bound DNA and enriching samples for target proteins without the use of a Doons homogenizer. , and contamination from other cell compartments is less than 15%.

Related products
Pierce™ Chromatin Preparation Module
Pierce™ ChIP Grade Protein A/G Plus Agarose
Proteinase K