Roswell Park Memorial Institute (RPMI) 1640 Medium was originally developed for suspension culture of human leukemia cell monolayers. RPMI 1640 medium has been found to be suitable for a variety of mammalian cells, including HeLa cells, Jurkat cells, MCF-7 cells, PC12 cells, PBMC cells, astrocytes, and cancer cells. We offer a variety of Gibco™ RPMI 1640 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
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Gibco™ RPMI 1640 medium has a unique effect compared to other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA, these ingredients are not available in Eagle's MEM and DMEM. In addition, the medium contains high concentrations of the vitamins inositol and choline.

Product Use
Research Use Only: Not intended for diagnostic or therapeutic use in animals or humans.

cGMP Manufacturing and Quality Systems
Gibco™ RPMI 1640 is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 does not contain proteins, lipids or growth factors. Therefore, RPMI 1640 medium requires additives, usually 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.