Thermo Fisher 303272 Each Dionex CarboPac PA1 -1 mmanalytical column (1 x 250mm)
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When running dual EGC mode on a Thermo Scientific™ Dionex™ ICS-6000 HPIC™ system equipped with Thermo Scientific™ Chromeleon™ CDS software, use a Thermo Scientific™ Dionex™ CarboPac™ PA1 (1 mm) column for monosaccharides and disaccharides. Predictable, high-resolution separations of certain oligosaccharides. Dual EGC mode can electrolyze potassium methanesulfonate and potassium hydroxide (KMSA/KOH) gradient eluent for use in series with Dionex KOH EGC 400 KOH eluent generator tank and Dionex MSA EGC 400 MSA eluent generator tank. Carbohydrate analysis. The use of dual EGC allows the sample set to be expanded to separate certain oligosaccharides as well as monosaccharides, disaccharides, and trisaccharides using electrolytically generated gradient eluents of potassium methanesulfonate and potassium hydroxide in combined concentration gradients up to 200 mM. Many separations require only isocratic separations. However, some analytes require step or gradient elution. Carbohydrate retention can vary with eluent concentration, and in some cases the elution order changes as eluent concentration increases.

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Paired with pulsed amperometric detection (PAD), this anion exchange column provides fast, reproducible separations without derivatization. 1 mm (standard bore/microbore) Dionex CarboPac PA1 for dual EGC, 1 mm columns contain the same resin as the proven 4 mm and 2 mm Dionex CarboPac PA1 columns for non-dual EGC carbohydrates Compound analysis.

Unique advantages of Dionex CarboPac PA1 columns for dual EGC

  • Using electrolytically generated potassium methanesulfonate and potassium hydroxide to analyze monosaccharides and disaccharides. Predicted high-resolution separations
  • Rugged, general-purpose column for determination of monosaccharides and disaccharides in a variety of matrices
  • The column's chemical structure is validated for Various official methods for food analysis (AOAC methods 995.13, 996.04, 997.08, 2000.11, 2000.17 and 2001.02)