Thermo Scientific™ NeutrAvidin Protein-HRP is a specially prepared form of avidin that reduces binding to peroxidase coupling conjugated biotin binding background for substrate-based detection.

Features include:
•Near-neutral isoelectric point—pI = 6.3, more neutral than natural avidin
• Almost sugar-free Kylated—Reduced lectin binding potential compared to native avidin
• No RYD recognition sequence—No known off-target binding domain, unlike streptomycin Same as avidin
• Economical—significantly less expensive than strepavidin

NeutrAvidin protein is deglycosylated natural avidin derived from egg whites. Excess carbohydrates are removed through a proprietary process, resulting in a protein with a more neutral isoelectric point and less non-specific binding properties. Purified and conjugated forms of NeutrAvidin protein provide excellent performance in Western blotting, ELISA and IHC applications requiring biotin-conjugated probes. NeutrAvidin protein assay specificity, sensitivity, and signal-to-noise ratio are generally comparable to or better than those of the much more expensive strepavidin.

Avidin is a glycoprotein found in the egg whites and tissues of birds, reptiles and amphibians. The biotin-binding protein contains four identical subunits with a combined mass of 67,000-68,000 daltons. Deglycosylation of avidin yields NeutrAvidin protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding can be reduced to undetectable levels, but biotin binding affinity is retained. NeutrAvidin proteins have the advantage of a neutral pI, which minimizes non-specific adsorption, while lysine residues remain available for derivatization or other custom couplings. NeutrAvidin protein has the lowest non-specific binding rate among known biotin-binding proteins. The specific activity of biotin binding is approximately 14µg/mg protein, which is close to the upper limit of theoretical activity.

Recommended applications: ELISA (relevant to assays), immunohistochemistry (relevant to assays), in situ hybridization (ISH) (relevant to assays), Western blot (relevant to assays) Related)