Purified calf intestinal alkaline phosphatase (CIP) in Tris buffer and 50% glycerol for protein Research methods. In molecular biology and protein research, alkaline phosphatase is mainly used to remove 5'-phosphate groups in DNA or as a reporter system for immunoassays such as ELISA. In ELISA, alkaline phosphatase is usually conjugated to a specific primary or secondary antibody, and activity can be detected with a chromogenic (or photogenerated) substrate.

Features of alkaline phosphatase:

•Purified—can be diluted and coupled without prior dialysis
•Concentrated b>—Approximately 20 mg/mL (report batch specific value)
•High specific activity—Typically above 1600 units/mg (report batch specific value)
•< b>Tris buffer—dissolved in a buffer containing 5 mM Tris, 5 mM magnesium chloride, and 0.1 mM zinc chloride, 50% glycerol,
pH ∼7.0

One unit The amount of protein required to hydrolyze 1 μmol of p-nitrophenyl phosphate (PNPP) per minute in glycine buffer (pH 9.6) at 25°C.