Thermo Scientific Pierce NBT and BCIP Substrates are for chromogenic blotting and IHC staining (using alkaline phosphatase probes) of nitroblue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate powder and ready-to-use solutions.

NBT (nitroblue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolylphosphate toluidine salt) are used in combination with alkaline phosphatase, a commonly used Antibody probe enzyme conjugate) produces an insoluble deep black purple precipitate when reacting. Pierce NBT and BCIP powders are available individually in two convenient, ready-to-use solutions (with and without levamisole inhibitors of endogenous phosphatase activity).

Features:

•AP Substrate—For detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
• Colorization—can be visualized without the use of special equipment; produces a purple-black precipitate that is easy to photograph
• Packaging Options—Select individual NBT and BCIP powders or preformulated ready-to-use solutions with or without levamisole inhibitors

Pierce NBT/BCIP 1-Step Solution (Product No. 34042) Advantages:
• Suitable for immunoblotting applications
• Ready-to-use single component
• Sensitivity at low background and minimizing inter-assay variability

< b>Pierce NBT/BCIP Plus Inhibitor 1-Step Solution (Product No. 34070) product advantages:
• Suitable for immunohistochemistry applications
• Ready-to-use single component
•Contains 1 mM levamisole to inhibit endogenous phosphatase activity that produces a dark purple precipitate in the presence of calf intestinal phosphatase

Related Products
1-Step™ NBT/BCIP Plus Inhibitor Substrate Solution
NBT substrate powder (nitrotetrazolium blue chloride)
BCIP substrate powder (5-bromo-4-chloro-3'-indolyl phosphate toluidine)