Thermo Scientific Pierce Metal Enhanced DAB Substrate Kit in a specially formulated diaminobenzidine peroxidase substrate Cobalt chloride and nickel chloride are used to produce darker colors in immunohistochemical staining.

Metal Enhanced DAB Substrate Kit Features:

•Incredible Sensitivity—50 times more sensitive than traditional DAB methods and more sensitive than other metal enhanced DAB methods 30x
• Low background, high intensity—Produces a friable, dark brown-black precipitate that is darker than the grey-brown precipitate produced using unenhanced DAB. And, even with increased intensity, background remains virtually non-existent
• Only two components—Just mix two liquid components and your working solution is ready to use
• 6 hour stability—The innovative working solution is stable for over 6 hours at room temperature, whereas other DAB substrates must be used immediately

To prepare the product, use Pierce Stabilized Peroxide Buffer before use The 10X metal-enhanced substrate solution is diluted with liquid. The special formula makes the 1X substrate stable at room temperature for at least 6 hours. There is no increase in background color of the substrate, and unlike typical metal-enhanced DAB methods, reactivity remains unchanged (1,2). The resulting precipitate is a dark brown-black color, darker than that produced by DAB alone. Not only does the intensity increase, but the background value also decreases, which increases the signal-to-noise ratio and makes screening tissue or membrane antigens easier.