Thermo Scientific SuperSignal ELISA Femto Substrate is specially formulated to achieve excellent protein detection results and is suitable for chemiluminescent ELISA applications. Detection of the low end of the linear range.

Features of SuperSignal ELISA Femto Substrate:

• Immediate Luminescence—Generates a strong signal immediately at room temperature or 37°C with a wavelength of 425nm
• Improved low-end linearity—Easily detect low-abundance proteins with a high signal-to-noise ratio and good low-end linearity of the dose response curve
• High sensitivity— Detect femtogram-level target protein in ELISA experiments
• Shorten detection time—High sensitivity can shorten ELISA incubation time
• Stable storage—at room temperature Store for 6 months, store at 4°C for at least 12 months, working solution is stable for 6 hours

SuperSignal ELISA Femto Substrate is a chemiluminescent substrate for enzyme detection and quantitative analysis. ELISA experiments or other solution-based detection experiments provide higher sensitivity. These ELISA reactions can be performed in test tubes or microplates, and relative light units (RLU) are detected by a photometer.

SuperSignal ELISA substrate uses an improved enhancer system to meet the needs of high-throughput screening (HTS) applications and diagnostic assays. SuperSignal ELISA Femto Substrate generates a detectable light signal within one minute with the addition of trace levels of soluble HRP, saving up to 30 minutes per assay. This property makes SuperSignal ELISA Femto Substrate ideal for HTS applications, capable of completing up to 100,000 analyzes on automated equipment.

Related resources:
Detection technology development and ELISA technical guidelines
ELISA Substrate Selection Guide

Related products:
SuperSignal ELISA Pico chemiluminescent substrate
Blocking buffer
coated microplates