Thermo Fisher 41105-159570XL Each Hypersil HS C8 reverse phase HPLC chromatography column,5 μm,50 mm x 150 mm
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Get maximum loading for your scale up from analytical to a prep LC method with Thermo Scientific™ Hypersil™ PREP HS C8 reversed phase preparative HPLC column. With similar selectivity to C18 columns and predictable elution order, C8 columns are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations.

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Column FormatPreparative Column, XtendedLife
Diameter (Metric)50 mm
Length (Metric)150 mm
Max. Pressure2000 psi (140 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area350 m²/g
Temperature60°C
USP TypeL7
Stationary PhaseC8
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load7%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
Unit SizeEach

Similar selectivity to C18 columns, but with lower retention

  • Successfully retains and separates moderately hydrophobic analytes.
  • Suitable for fatty acids. Fatty acids are too hydrophobic and are not suitable for analysis using C18 columns.
  • C8 (USP L7) Selective – High surface area particles with 100Å pore size.
  • Stable at pH values 2 to 8.
  • The upper limit temperature is 60℃.

Fast separation

  • Have higher analytical throughput compared to C18 alkyl chain chemical structure.
  • Compounds elute more quickly due to reduced hydrophobic interactions.

Preparative HPLC column hardware options - Based on column inner diameter (id) and column performance requirements.

Standard threaded and standard flange hardware

  • Designed for standard applications, professionally packaged and rugged, providing a universal product for routine use .
  • Standard threaded hardware for use with columns id < 40 mm.
  • Standard flange hardware for columns id ≥ 40 mm.
  • Standard hardware can be modified as required to be SFC compatible.

XtendedLife Hardware

  • Improve column life and secure media bed with built-in Dynamic Axial Compression (DAC) system Keep it tightly packed all the way through.
  • Compatible with SFC, inner diameter up to 50 mm.

Preparative protection equipment has proven to be an effective and cost-effective way to extend the life of preparative columns. Our preparative guard columns require the use of preparative guard cartridges.