The Dionex CarboPac MA1 column can also analyze neutral monosaccharides released by exoglycosidases. Monosaccharides are removed from the column voids, allowing immediate analysis of contaminating exoglycosidase activity. Using a simple hydroxide eluent, Dionex CarboPac MA1 columns can separate analytes isocratically or with gradient elution. The low flow rate ensures low eluent consumption and reduces errors related to eluent preparation.

Unique selectivity for analysis of sugars, sugar alcohols and aldoses

• No need for sample derivatization
• Higher sensitivity than differential refractive index Detection
• Simple method using pulsed amperometric detection (PAD)
• Compatible with room temperature separations
• Batch reproducibility
• Long lifetime

About the Dionex CarboPac Column Series
The Dionex CarboPac Series offers a selection of high performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. . Paired with PAD that can detect carbohydrates without derivatization, these columns enable simple and reliable separations of sugar alcohols, mono- and disaccharides, polysaccharides and oligosaccharides.

Dionex CarboPac columns provide high-resolution separation of:

• Glycoprotein oligosaccharides and glycoconjugates in dietary fibers, including fructans, maltodextrin, and amylopectin< /li>
• Monosaccharides, such as those obtained from glycoprotein hydrolysates and plant hydrolysates
• Monosaccharides and disaccharides