NA-Fluor™ Influenza Virus Neuraminidase Assay Kit provides validated reagents and Standardized protocol for performing neuraminidase assays, including neuraminidase inhibitor (NI) sensitivity screening using the fluorescent MUNANA substrate assay.

Main Product Features:
•Efficient design—Neuraminidase assay in one complete kit
•Optimized formulation—Detection reagents have been tested according to the NISN protocol Optimized for ideal performance
• Robust application—detection of NI resistant virus functionality in mixed viral populations
•Easy to use & flexible screening—stable fluorescent signal enables batch mode or high-throughput processing

Neuraminidase Assay in One Complete Kit
NA-Fluor™ Influenza Virus Neuraminidase Assay Kit includes titrated viruses based on neuraminidase activity isolates and a comprehensive protocol for performing neuraminidase inhibition assays. The assay can also be used to monitor neuraminidase activity from non-viral or bacterial sources. The NA-Fluor™ Kit contains reagents for 10 96-well microplates, sufficient for a total of 960 assay well assays, and includes the following components: Fluorescent MUNANA Neuraminidase Substrate, Assay Buffer, and Buffer for Enhancement and Stop solution to stabilize signal. The assay was performed in a standard black microplate (not provided with the kit) and read on a standard fluorometer.

Neuraminidase assay versus NISN protocol
Optimization of the NA-Fluor™ detection reagent formulation to be comparable to several established MUNANA-based assay protocols, e.g. :
• MUNANA substrate concentration, assay buffer formulation, and assay conditions are consistent with the NISN IC-50 assay protocol.
• Data generated using the NA-Fluor™ Assay Kit are consistent with data generated using the established MUNANA-based protocol.
Based on these key parameters, investigators were able to compare data obtained in the current NI Tolerance Monitoring Screen using the NA-Fluo™ Assay Kit with data obtained using the previous MUNANA assay protocol (Figure 1).

Detection of NI-resistant viruses in mixed virus populations
When detecting sensitive and oseltamivir-resistant viruses using the NA-Fluor™ Assay Kit, IC- A large change in the 50 value allowed the mutant virus to be detected in the mixed virus sample (Figure 2). This ability is critical for detecting resistant viruses in NI susceptibility surveillance of clinical isolates of mixed resistant and susceptible viruses.

Flexible screening of neuraminidase activity
NA-Fluor™ assay for screening of few to hundreds of viral isolates or for high-throughput lead discovery of thousands Process compound screening provides a simple, flexible format with high confidence levels of quality data. The assay has excellent performance, showing a Z´ of 0.78 - 0.8, making it suitable for high-throughput screening. Once the assay is complete, the fluorescent reaction products remain stable at room temperature for several hours, allowing for flexibility in read times and data comparability from the first microplate to the last microplate. The assay signal was nearly constant and the IC-50 values (data not shown) were the same for data collected up to 4 hours at room temperature and up to 4 days at 4°C after the end of the assay (Figure 3). The NA-Fluor™ assay was optimized to be run as an endpoint assay at 37°C after NI preincubation. However, MUNANA substrate turnover rates remain linear with viral neuraminidase over 2 hours, allowing the assay to be performed in only 20 minutes to save time or up to 2 hours to increase signal output. For researchers wishing to develop assays or monitor substrate turnover in the presence of inhibitors, the assay can also be performed in real time without the addition of stop solution (Figure 4).

For scientific research use only. Not intended for diagnostic use.