RNaseAlert™ Laboratory Test Kit v2 detects RNase activity with a convenient and sensitive fluorometric assay that provides real-time result. This kit is suitable for testing small amounts of samples and ensuring that solutions, test tubes, tips, etc. in the laboratory are free of RNase. This new version contains a substrate that is more sensitive (∼60%) than the previous version. This kit contains enough reagents for 25 reactions.

• Detect as low as ∼0.3 pg of RNase A using a fluorometer
• Simple and straightforward 30-minute assay

RNaseAlert™ Laboratory Test Kit v2 uses a new and highly sensitive A new type of higher RNA substrate, which is labeled with a fluorescent reporter molecule (fluor) on one end and a quenching group on the other end. In the absence of RNase, the physical proximity of the quencher suppresses the fluorescence in the fluor to very low levels. However, when RNase is present, the RNA substrate is cleaved and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be easily detected with filter or monochromator based fluorometers. Because the fluorescence of RNaseAlert™ substrate gradually increases in the presence of RNase activity, results obtained using fluorometer monitoring can be evaluated kinetically. The sequence of RNaseAlert™ Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, Micrococcal Nuclease, S1 Nuclease, Mung Bean Nuclease, and Benzonase™.

Fast and Convenient Protocols
The RNaseAlert™ program takes only minutes to set up. Lyophilized fluorogenic substrate is reconstituted in the provided tube and incubated with detection solution for several minutes. The results are then read using a fluorometer. Samples that fluoresce significantly compared to negative controls are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided as controls.