If you want to covalently couple antibodies, peptides, intact proteins, and functional enzymes to the surface of magnetic beads, please Choose Dynabeads™ Tosylactivated. Optimized antibody orientation makes these beads ideal for immunoprecipitation of proteins and protein complexes.

Ideal for immunoprecipitation of protein complexes:
Low background and covalent attachment of antibodies to the bead surface make Dynabeads Tosylactivated ideal for immunoprecipitation of proteins and protein complexes ( Excellent choice for co-immunoprecipitation, co-IP). Gentle and rapid magnetic aggregation of beads and extremely short incubation (possibly due to fast surface binding kinetics) make Dynabeads Tosylactivated ideal for immunoprecipitation of extremely unstable or and/or transient (ephemeral) protein complexes.

Dynabeads Tosylactivated Microbeads Surface Properties:

• p-Tosyl-sulfonyl (Tosyl) group
• Hydrophobic, pH neutral
• Covalent binding via primary amine (NH₂) or sulfhydryl (SH) groups

Ultrafast protocol:
• Identification of protein complexes Member times are measured in minutes rather than hours
• Short separation times are sufficient to identify transient and unstable complexes
• Identify binding partners that would not be identified with longer protocols
• Rapid protocols enable already extremely low background Binding is further reduced
• Greater signal-to-noise ratio

Applications:
• IP of proteins and protein complexes
• Coupling of functional enzymes to micro Bead surface for downstream analysis
• Couple peptides to bead surface to identify binders

Purify peptides, proteins and enzymes with:
• Transient Stable
• Complete structure
• Poor temperature stability
• Has native configuration and complete functions

Overview of coupling process:
By overnight incubation Optional ligands and Dynabeads Tosylactivated achieve covalent coupling. Ligands coupled to Dynabeads Tosylactivated typically include peptides and proteins (such as antibodies used for immunoprecipitation or co-immunoprecipitation). Coupling occurs at neutral to high pH and 37°C. We recommend coupling at pH 8.5-9.5, but for ligands with poor pH stability, coupling can be performed in other buffers at pH 7.4.

Once the ligand coupling step is complete, the actual surface coating of Dynabeads Tosylactivated is no longer active and therefore non-specific binding is low.

Binding capacity per milligram of beads:
Varies with ligand (e.g. 14-19µg IgG)

About Dyanbeads: b>
Dynabeads are nonporous monodisperse superparamagnetic microbeads. They are highly mobile in solution, allowing ligands coupled to the beads to continuously interact with the entire sample. Transfer the test tube to a tube rack containing a strong magnetic field, and the superparamagnetic beads will adsorb to the tube wall. The strong magnetic field quickly adsorbs the beads to the tube wall, so all the supernatant can be easily removed by pipetting. The washing steps are similar.