Proteins can be rapidly purified using ion exchange chromatography using the sulfonic acid stationary phase of a Thermo Scientific™ ProSwift™ SCX-1S LC column. These ion exchange monolithic columns leverage the superior resolving power of non-porous analytical media with the high capacity benefits of traditional porous media. They achieve excellent separations using fast gradients at both low and high flow rates, thereby increasing productivity. These columns are both rugged and robust, demonstrating stability and reproducibility even after completing hundreds of runs.