Highly sensitive mono- and disaccharide analysis in therapeutic glycoprotein, food and beverage applications

• High-efficiency analysis with good resolution between adjacent peaks
• Fast analysis time of less than 10 minutes
• Low eluent consumption
• Optimized resolution between glucosamine/galactose and glucose/mannose peak pairs
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• Subtle selectivity difference from Dionex CarboPac PA1 or PA10 columns; ideal for separating difficult-to-distinguish peak pairs

Dionex CarboPac PA20 columns consist of a 6.5µm diameter, functionally covered Thermo Scientific™ Dionex™ MicroBead™ resin consists of fine latex, non-porous microbeads. This thin-film-coated resin structure provides excellent mass transfer, enabling high-resolution chromatography methods and rapid re-equilibration.

Available in multiple formats for flexibility for method development

• Dionex CarboPac PA20 analytical columns (3 x 150 mm) are available in monosaccharides or disaccharides. High-resolution separation without derivatization.
• Dionex CarboPac PA20 capillary column (0.4 × 150 mm) offers higher mass selectivity, lower sample volume requirements and significantly lower solvent usage.
• Dionex CarboPac PA20 Fast Sialic Acid Column (3 x 30 mm) accelerates sialic acid determination, completing sample analysis in less than 5 minutes.

About the Dionex CarboPac Column Series
The Dionex CarboPac Series offers a selection of high performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. . Paired with PAD, which is capable of detecting carbohydrates without derivatization, these columns enable simple and reliable separation techniques for sugar alcohols, mono- and disaccharides, polysaccharides and oligosaccharides.

Dionex CarboPac columns provide high-resolution separation of:

• Glycoprotein oligosaccharides and glycoconjugates in dietary fibers, including fructans, maltodextrin, and amylopectin< /li>
• Monosaccharides, such as those obtained from glycoprotein hydrolysates and plant hydrolysates
• Monosaccharides and disaccharides