DynaGreen Protein A magnetic beads enable high-performance direct and indirect immunoprecipitation while being an environmentally sustainable option. DynaGreen Protein A magnetic beads are microplastic-free, sub-micron magnetic beads. Magnetic beads provide reproducible isolation of target proteins in a simple and efficient workflow that can support manual protocols or be automated on KingFisher instruments.

Features include:
• Produces reproducibly high yields and high-quality immunoprecipitation
• Reduces the environmental impact of research
• Improves purity and reduces non-specific binding
• Easily expand and automate operations
• Porous magnetic beads reduce antibody usage

Manual separation is simple, gentle and efficient
DynaGreen Protein A magnetic beads are free of Microplastic superparamagnetic beads with recombinant Protein A covalently coupled to the surface. The smaller size of submicron magnetic beads (approximately 250 nm) reduces the sedimentation rate and increases the target capture surface area. Therefore, efficient and high-yield separation of target proteins can be achieved within 80 minutes in a simple direct or indirect immunoprecipitation (bind-incubate-elute) protocol without pre-wash steps.

The magnetic separation technology used by DynaGreen Protein A magnetic beads is fast and gentle, producing very little physical pressure on the target protein. Additionally, the non-porous nature of the magnetic bead surface means there is a lower risk of antibodies becoming stuck and becoming inaccessible, minimizing the amount of antibody required to produce accurate and reproducible results. The isolated high-yield and high-purity target proteins are highly compatible with downstream immunoblotting or mass spectrometry.

The following product manual includes a list of required reagents and materials as well as details on compatible buffer formulations.

Fast and reproducible automated workflows
As experimental scale increases to mid- to high-throughput, DynaGreen Protein A magnetic bead workflows can be seamlessly scaled using the KingFisher automation platform . The automated protocol takes only 40 minutes to run and can process up to 96 samples per run, significantly reducing hands-on time while maintaining the same reproducibly high target yield and low non-specific binding as the manual protocol.

A certified choice for environmentally sustainable research
Microplastic-free DynaGreen Protein A magnetic beads are designed with both improved performance in immunoprecipitation workflows and envirnmental factor. Microplastic-free DynaGreen Protein A magnetic beads have been shown to help deliver high purity and yield of target proteins in both direct and indirect immunoprecipitation workflows compared to other magnetic bead alternatives. At the same time, the product has a large number of sustainable product design elements certified by the third-party ACT label by combining the principles of green chemistry and green engineering. Choosing DynaGreen Protein A magnetic beads can help reduce the environmental impact of your research without compromising your results.