MagniSort™ Human CD4 2 Step Enrichment Kit is designed to screen for positive selection after first depletion of monocytes CD4+ T cells were magnetically isolated. It has been optimized for the isolation of CD4+ T cells from human peripheral blood mononuclear cells using biotinylated anti-human CD14 and anti-human CD4 antibodies and streptavidin-coated magnetic beads. In the first step, monocytes are bound by anti-human CD14 antibodies and then by magnetic beads. When placed in a magnetic field, the monocytes were immobilized in the magnet, while the remaining cells were poured out for use in the second step. In the second step, monocyte-depleted cells are bound to anti-human CD4 antibodies and then to magnetic beads. Now, when placed in a magnetic field, the CD4+ T cells were immobilized in the magnets, while the unwanted cells were poured out.

After enrichment, the purity of selected cells can be verified by staining with anti-human CD4, clone OKT4 or SK3.

Reported Applications
Magnetic Cell Separation