Roswell Park Memorial Institute (RPMI) 1640 Medium was originally developed for suspension culture of human leukemia cell monolayers. RPMI 1640 medium has been found to be suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and cancer cells. We offer a variety of Gibco™ RPMI 1640 modified media for a wide range of cell culture applications. Use Base Selection ToolFind a suitable recipe.


The reason why Gibco™ RPMI 1640 medium is better than other media It has a unique effect because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA, ingredients not found in Eagle Minimum Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the medium contains high concentrations of the vitamins inositol and choline.

Intended Use of Product
Research use only. Not for use in animals or humans for diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
The Gibco™ RPMI media is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 medium does not contain proteins, lipids or growth factors. Therefore, RPMI 1640 medium requires supplements, usually 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.