GeneArt™ Seamless Cloning and Assembly Enzyme Mix can convert 1 to 100% of any sequence from 1 to Four PCR fragments are directionally cloned simultaneously into any linearized vector. GeneArt™ Seamless Enzyme Mix is an economical choice for creating constructs up to 13 kb with options for high-throughput assembly.

•Efficiency: Pre-clones can be selected for large fragments to improve cloning efficiency
•Quick and easy: Can be cloned in a single vector (up to 13 kb ); no restriction enzymes, ligation, or recombination sites required
•Free Tools:Use our freeWeb tool to design your final construct and DNA oligonucleotides through in silico
•Vector flexibility :Use our linear vectors or a vector of your choice

For cloning where the pre-existing DNA elements are too long to amplify by PCR and where the final molecule is up to 40 Kb, consider Use GeneArt™ Seamless PLUS Cloning and Assembly Kit (1–4 DNA fragments only); for final molecules For larger than 110 kb, please consider using GeneArt™ High-Order Genetic Assembly System.

Simple and fast clone creation
GeneArt™ Seamless Cloning is a simple two-step process that includes in vitro assembly followed by transformation. This enzyme mix uses a proprietary enzyme/buffer mix to assemble DNA fragments with no additional sequence or traces ("seamless"). Terminal homology, if desired, can be easily incorporated by PCR amplification using custom DNA oligonucleotides (designed through our free web tools).

Efficient, flexible and accurate cloning
Cloning success is independent of insert sequence and vector type, you can design and add almost any desired sequence or sequence combination into any plasmid, provided the plasmid can be linearized by restriction enzyme digestion or PCR. The circular clone obtained from the reaction contains only your original vector sequence, insert, and specified homology, with no extraneous nucleotide insertions.

In silico cloning design support
A critical step in GeneArt™ seamless cloning is the correct design of fragments and oligonucleotides with appropriate homology and spacing, to help ensure successful cloning assembly. To simplify and accelerate the design process, we offer a free online tool (GeneArt™ design for seamless or high-order assembly and mutation Tools) to help you design experiments through computer simulations. The tool guides the user through construct design, including fragment introduction, order, and orientation; examines regions of homology and potential design issues; and, if necessary, creates primers for precloning or incorporates interfragment end homologies in together; and generate final construct map files and Genbank-specific sequences for download or import into Vector NTI™ software.

Applications
GeneArt™ Seamless Cloning and Assembly Enzyme Mixes are designed for cloning and DNA assembly in a variety of molecular and synthetic biology applications and beyond. The product can be used to create modular expression vectors with interchangeable parts and can be used to perform a variety of tasks that might otherwise involve multiple steps. Use mixtures to construct fusion proteins; delete, replace, or add DNA elements such as restriction sites in existing vectors; and perform a variety of other techniques that require manipulation of genetic sequences.

Research use only. Not intended for therapeutic or diagnostic use in humans or animals.