Compared to poly-A or cap-binding methods that introduce bias and select only part of the transcriptome, Low Input RiboMinus™ Eukaryotic Cell System v2 provides truly complete transcriptome isolation for next-generation sequencing analysis by selectively removing up to 99.9% of 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA.

System features include:

•Magnetic bead purification for fast, seamless purification
•1 hour workflow
•Low input range 100 ng–1 µg

Low Input RiboMinus™ Eukaryotic Cell System v2 outperforms previous systems (RiboMinus™ Eukaryotic Cell Kit for RNA-Seq) by removing significantly more rRNA (cytoplasmic and mitochondrial) from total RNA Improve on the basis of. When used for sequencing with our library construction kits, the Low Input RiboMinus™ Eukaryotic Cell System v2 provides a greater degree of reference database mapping with less bias, maintaining relative gene expression levels of smaller RNA species. The resulting total RNA after removal contains less contaminating rRNA, reducing variability.

Low Input RiboMinus Eukaryotic Cell System v2 is designed to remove human, mouse and rat rRNA. Contains a magnetic bead purification module for purifying the entire transcriptome without altering the expression levels of small or non-coding RNAs. For column purification, see RiboMinus™ Eukaryotic Cell Kit v2. Low Input RiboMinus Eukaryotic Cell System v2 has an RNA input range of 100 ng–1 µg. For high starting amounts (up to 5 μg), the RiboMinus™ Eukaryotic Cell System v2 is recommended.

Low Input RiboMinus™ Eukaryotic Cell System v2 contains sufficient reagents for 12 ribosomal RNA depletions.