PureLink™ Microbiome DNA Purification Kit can be used to purify DNA from a variety of sample types, including challenging samples such as feces and soil. Rapidly purify high-quality microbial and host DNA. The kit uses proven PureLink spin column technology to enable robust production of purified DNA that can be used directly in downstream PCR, sequencing, or other applications. The highly efficient triple lysis method, rapid inhibitor removal, and versatility make this kit the ultimate kit for microbiome research projects and projects aimed at rapid detection of pathogenic bacteria in a variety of samples.

PureLink Microbiome DNA Purification Kit product features include:

•Effectively lyses all microorganisms, including those with thicker and larger Tolerant species with complex cell walls)
• Elimination of inhibitor compounds by precipitation using novel purification buffers
• Simplified protocol suitable for many biological samples
• High purity DNA recovery with common downstream applications such as qPCR and next-generation sequencing)

One kit for isolating microbial and host DNA from a diverse set of sample types
PureLink Microbiome DNA Purification Kit minimizes The need to order a single kit for a specific sample type as the kit is optimized for use with a wide range of biological samples.

This kit enables microbial—and (if applicable) host—DNA purification from the following samples:

• Feces
• Urine
• Saliva
• Swabs (vaginal, oral, skin, rectal, environmental)
•Transport media
•Growth media
•Soil

Analysis of the human microbiome< br>There are 100 trillion bacteria, archaea, fungi, protists and viruses in the human body, which have an important impact on our health. The term "microbiome" refers to the variety of different microorganisms within these communities. Currently, their genes are the most direct way to identify these organisms. Deviations in the composition of a healthy microbiome are associated with many human diseases, including inflammatory bowel disease, obesity, cancer, asthma, diabetes and allergies.

Purification of aggregated microbial DNA from biological samples is a critical step in understanding how the microbiome affects human health. It is critical that the recovered DNA accurately reflects the expression of the diverse microorganisms in the sample community. The mechanical disruption and triple lysis methods used in the PureLink Microbiome DNA Kit help ensure that microbial DNA can be recovered from the most difficult samples, such as Gram-positive bacteria found in stool—to identify the full range of microorganisms present.