Thermo Fisher A30868 1 x 1 mL TaqPath ProAmpMultiple master mix
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A30868 1 x 1 mLTaqPath ProAmp Multiplex Master Mix

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TaqPath ProAmp Multiplex Master Mix is suitable for higher multiplex amplification (up to 4 targets) in high-throughput genotyping and copy number variation protocols that require stringent reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It provides sensitive, reliable results for genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Multi-Path Master Mix is produced under a strict ISO13485 quality management system and is designed to produce products with consistent performance from batch to batch.

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Two TaqPath ProAmp Master Mix Formulations for Different Throughput Needs
TaqPath ProAmp Master Mix—Contains ROX inert reference dye for low-pass detection of up to three targets
•TaqPath ProAmp Multipass Master Mix—Contains MUSTANG PURPLE inert reference dye for higher multi-pass detection of up to four targets

Advantages of TaqPath ProAmp Master Mix
Excellent data quality—Genotyping and copy number determination with high specificity, dynamic range*and reproducibility even in the presence of inhibitors
Available Resistant to inhibitors commonly found in samples prepared from human or animal origin (oral mucosal swabs, blood and card sampling)
• Proprietary Dual Lock Taq DNA hot-start mechanism enables automated workflows< b>72-hour PCR front-bench stability
Multi-pass amplification range—Two formulations enable detection of single to four targets per reaction
Excellent manufacturing consistency—Produced in an ISO 13485 certified facility
Compliance Pack contains the most commonly requested compliance documents and is available below
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Highly accurate genotyping using purified or crude lysate loading
In situations where small amounts of sample are available or PCR inhibitors are unavoidable (e.g. in clinical diagnostic testing center), accurate genotyping is very important. TaqPath ProAmp Master Mix has been optimized to provide accurate and reproducible results even in low-volume reactions prepared using purified or crude lysate sample input. The graph below shows the genotyping accuracy of the same set of TaqMan genotyping assays using crude lysates from blood samples compared to competitor mixtures. TaqPath ProAmp Master Mix has been optimized to provide highly specific allelic discrimination, as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High-Accuracy Copy Number Quantification
The versatile TaqPath ProAmp Master Mix also enables high-accuracy determination of copy number variations using purified genomic DNA samples. The graph below shows the copy number variation results using TaqPath ProAmp Master Mix compared to two other mixes. TaqPath ProAmp Master Mix can be used to accurately determine copy number in samples containing up to four copy numbers.

Universal Reagents Manufactured to cGMP Standards
TaqPath Master Mixes are universal reagents, labeled "for laboratory use", ISO 13485 certified and FDA registered in compliance with cGMP Produced in standard factories.

Compliance Pack
To effectively support your compliance needs, the most commonly requested compliance documents are available here:
• With TaqPath Master Mix Current Good Manufacturing Practice (cGMP) followed statement.PDF">Quality Statement
• Detailed batch-specific Certificate of Inspection (COA) (see "Documents and Downloads" section below)
Product Stability Statement
• Supplier Assessment Support
-- CompletedSelf Assessment
-- ISO of the manufacturing site Certificates
• For inquiries regarding EU REACH compliance, please email LSG ChemicalCompliance@thermofisher.com
Technology The support teamis here to help.

Learn more about multipath assays, TaqPath probes, and how our products can help your project ›

&ast; Dynamic range is a measure of the concentration of template in the method and sample, as well as the master mix A property of the recipe; therefore each result may vary.