MagMAX Cell-Free Total Nucleic Acid Isolation Kit is designed to enrich nucleic acid species present in cell-free biological samples such as plasma . The kit uses MagMAX magnetic bead technology to ensure reproducible recovery of high-quality nucleic acids suitable for a variety of applications including real-time PCR, digital PCR and next-generation sequencing.

Magnetic bead purification formats allow you to easily handle a variety of sample starting volumes (1 mL to 6 mL). When used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor, 6 to 24 plasma samples can be separated at one time from a starting volume of 2 mL. Alternatively, samples can be processed manually using a magnetic stand.

Features of the MagMAX Cell-Free Total Nucleic Acid Isolation Kit include:
• Automated, phenol-free extraction
• Fast procedure that allows for use with KingFisher Duo Prime or KingFisher Flex with 24-well deep well head Process 6 to 24 samples in 90 minutes or less when used with the Magnetic Particle Processor
• Flexible solution, accommodating starting sample volumes from 1 L
to 6 mL of plasma
• No carrier RNA required
• Elution volumes range from 15 μL to 60 μL
• Compatible with Oncomine Lung cfTNA Research Assay

Go beyond cell-free DNA and get a holistic pictureIsolation And analysis of circulating cell-free total nucleic acids (cfTNA) in plasma or cell-free samples can be used as a non-invasive method to detect and monitor targets associated with certain disease states such as cancer.

The first step in obtaining this valuable information is efficient nucleic acid isolation, which specifically recovers circulating nucleic acid species that contain information relevant to disease states. The importance of cell-free DNA (cfDNA) is establishing, but it may not tell the whole story. Cell-free RNA (cfRNA) sequences as key biomarkers as well as microRNA and gene fusion forms. This combination of cfRNA and cfDNA provides researchers with opportunities to expand research and advance human health.

MagMAX Cell-Free Total Nucleic Acid Kit Developed by the same Thermo Fisher Scientific scientists, we provide you with the MagMAX Cell-Free DNA Isolation Kit. The difference is that all cfRNA species are included in the eluate, allowing you to obtain more information from a single cell-free sample. Like the cfDNA isolation kit, the cfTNA kit is designed for manual and automated isolation of nucleic acids from a wide starting sample volume range of 1 to 6 mL. The phenol-free, magnetic bead format makes the kit fully suitable for automated separations.

The scalable format allows for a variety of sample input amounts, so the number of samples processed with a single kit can be changed. For example, provide enough reagents to process 50 samples containing 2 mL of plasma each. Alternatively, 25 samples each containing 4 mL of plasma can be processed using the same kit. With higher and lower starting volumes, the number of samples that can be processed changes accordingly. This unique product attribute allows you to tailor your product to your specific needs

Advantages of MagMAX Magnetic Bead Purification
Magnetic beads offer many advantages over other technologies for isolating nucleic acids. Magnetic beads bind DNA more efficiently than glass fiber filter columns, resulting in higher and more consistent yields. Additionally, since no filter columns and vacuum manifolds are used, there is no risk of cell particles in the sample clogging the filter column. This clogging problem is particularly severe for protein-rich bulk samples such as plasma that are frequently used for circulating cell-free nucleic acid studies.