Ambion™ LeukoLOCK™ Isolation & Stabilization Kits are two kits containing a total RNA isolation system (patent pending) One, this system is an innovative method for isolating whole blood cells and stabilizing and extracting total RNA from leukocyte populations. This kit contains enough reagents for 20 reactions. The LeukoLOCK™ System (SKU #AM1923) is optimized for use with human blood. Blood is a repository of cellular information; however, the presence of globin mRNA in RNA prepared using whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ system uses filter column-based leukocyte removal technology to separate leukocytes from whole blood and Ambion RNAlater™ solutions to stabilize the cells on the filter column. By excluding red blood cells, RNA purified from captured leukocytes is inherently free of globulin mRNA, improving sample utility for expression profiling and other applications (see figure).

LeukoLOCK™ Isolation & Stabilization Kit
Pass a 9–10 mL sample of anticoagulated blood through a LeukoLOCK™ filter column, which captures the total leukocyte population while removing red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline, rinse the filter column with RNAlater™Solution to stabilize RNA in captured leukocytes (see protocol diagram ). RNA can be isolated immediately, or stable cells can be left at room temperature for several days or longer at –20°C or –80°C (see figure).

Ancillary Products:
The LeukoLOCK™ Isolation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (SKU #AM1934). These kits are available separately to accommodate blood sample collection at one site and sample processing at another site, but can be purchased together as the LeukoLOCK™ Total RNA Isolation System (SKU #AM1923).