Ambion™ RNA-Grade RNase T1 is optimized for researchers conducting RNA structure, RNA sequencing, protein footprinting and boundary experiments. Cleaves 3' of single-stranded G residues and is tested for purity to ensure there is no contaminating ribonuclease activity that cleaves unintended sites. Supplied in single tubes of 200 µL each (1U/ µL). RNase T1 can be used in boundary experiments to identify very small RNA sequences required for selective activities such as protein binding or catalysis. In addition to RNA structure analysis applications, RNA-grade ribonucleases can also be used to locate binding sites on RNA by comparing cleavage patterns in the presence/absence of RNA-binding proteins.

Unit definition:
100 units refers to the enzyme required to increase the absorbance at 260 nm by 0.01428 units per minute using 60 µg/mL yeast total RNA as a substrate at room temperature. quantity.

Companion Products:
Other available RNA-grade ribonucleases include RNase A (SKU# AM2274) 3' that cleaves single-stranded C and U residues. A combination of single- and double-strand-specific ribonucleases allows rapid analysis of the physical structure of target RNA.