When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for Add other chemical groups. Modified dUTP can be incorporated by reverse transcriptase. Aminoallyl modification enables downstream reactions with amine-reactive compounds such as N-hydroxysuccinimide (NHS) ester; therefore aminoallyl-modified cDNA can be labeled with any amine-reactive group.

Use this modified nucleotide:
Use 5-(3-aminoallyl)-dUTP as recommended in your reverse transcription protocol. It may be useful to use 5-(3-aminoallyl)-dUTP instead of 30–80% dTTP, but the exact ratio will depend on the experimental goals.