Click-iT™ GlcNAz Metabolic Glycoprotein Labeling Reagent provides a simple and reliable two-way The first part of a step-by-step technique that can be used to identify and characterize intracellular O-GlcNAc glycoproteins. In the first step, azide-modified glucosamine (GlcNAc) is used to incubate with cultured cells. Azidosaccharides are incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive properties of the oligosaccharide biosynthetic pathway. In the second step, the Click-iT™ Glycoprotein Detection Kit is used to detect gels (TAMRA or Dapoxyl™ Alkynes) or Western blots (Bio azide-labeled glycoproteins. Click-iT™ glycoprotein products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analysis of glycoproteomics.